Behavior of Calliphora vicina (Diptera, Calliphoridae) Under Extreme Conditions


 

J. Faucherre,1,2 D. Cherix,1,2,4 and C. Wyss3

KEY WORM: Calliphora vicina; blownies; forensic entomology; oviposition; activity.

If a dead human body is left exposed above the ground it will attract blowflies, which will rapidly lay eggs. The maggots found may contrebute to police inves­tigation in several ways. One of them is the estimate of postmortem interval (PMI) (see Greenberg, 1991; Erzinçlioglu, 1996). But as discussed by different authors (e.g., Erzinçlioglu, 1996), we are missing data about several aspects such as flight activity and egg-laying behavior under extreme conditions.

Temperature is one of the most important factors affecting fly oviposition activity and rate of larval development. It is generally accepted that activity of necrophagous flues stops below an air temperature of 10°C (Williams, 1984) or 12°C (Erzinçlioglu, 1996). Nevertheless, some authors showed that during the cold season, sun is probably more important than ambiant air temperature (see Green, 1951; Nuorteva, 1965; Greenberg, 1973). Deonier (1940) indicated that Calliphora spp. were active ai a minimum of 1.7°C with the usual minimum being between 4.5 and 10°C. Nuorteva (1959) found that Calliphora vicina and C. vomitoria were active when exposed to sun with an air temperature mea­sured in the shade of 5-6°C (a typical winter situation). Moreover, during win­ter, most of the captured species belongs to the genus Calliphora (Wyss, 1997; C. Wyss, personal observation). Some of these species seem more cold-adapted than species of Lucilia. Meyer and Schaub (1973) demonstrated that Calliphora species had a higher metabolic rate for identical temperatures compared to warm­adapted species (see also Greenberg, 1991). According to Greenberg (1991) this

‘Museum of Zoology, Palais de Rumine, P.O. Box 448, 1000 Lausanne 17, Switzerland.

2 Institute of Ecology, University of Lausanne, Biology Building, 1015 Lausanne, Switzerland. 3Criminal Police, Centre Blécherette, 1014 Lausanne, Switzerland.

4To whom correspondence should be addressed. Fax: 41 21 316 34 79. e-mail: daniel.cherix@ie­zea. un il .ch.

687

 

could be responsible for the lower-limit activity of Calliphora (2.5-4°C) com­pared to l.ucilia sericata (10-12.5°C). But flight activity and oviposition are also dependent on other factors. Generally, blowflies are not active and do not lay eggs at night or during bad weather (Greenberg, 1985; Catts, 1992; Erzinçlioglu, 1996). Nevertheless, some species have been found to lay eggs during the day in shady or dark places such as chimneys and cellars (Erzinçlioglu, 1996) or in car trunks (Greenberg, 1990). Observations made by Green (1951) and Greenberg (1990) showed, however, that some species such as L sericata, C. vicina, and Phormia regina could be active at night.

The literature about the influence of temperature on larval development is quite abundant, but there are surprisingly few studies on egg-laying and larval development at low temperatures. It has been said that eggs of C. vicina will not hatch at or below 4 ‘C (Nielsen and Nielsen, 1946). But Davies and Ratcliffe (1994) indicated that complete larval development was possible at 5°C and that embryo­genesis started even at 3.5°C. This research was conducted under laboratory con­ditions where flics were allowed to oviposit at higher temperatures (17-20°C).

We report here a case where C vicina was able to fly and oviposit under extreme conditions.

The body of a 77-year-old male was discovered on 25 July 1997, 18 days alter being reported missing, in a 10-m-deep cave located in a forest, in the Swiss Jura mountains at an altitude of 1260 m. He was tying on his back and dressed without visible injuries. The location of the body in the cave was in total darkness and the ambient temperature was constant (5°C). Following the local investigations made by the police, the body was carried to the Institute of Forensic Medicine (University of Lausanne) and kept for 3 days in a cold room (4°C) until postmortem investigations started. At that time, we collected several batches of eggs (a total of about 70) on the top of the head and in the mouth. Reared under laboratory conditions (26°C, 70% RH), the first eggs hatched about 6 h later and all reached the adult stage (see Fig. 1) and belonged to C vicina, a common blowfly in Europe (Rognes, 1991). The autopsy of the corpse could not estimate the day of death but revealed that death was due to an epidural ematoma subsequent to the downfall.

In order to demonstrate that blowflies were able to fly and lay eggs in this cave and to estimate the PMI, on 12 August (Day 1, fig. 1) we placed, at the previous body location, 1.5 kg of fresh meat and 1 kg of pork liver. We also recorded temperatures close to the meat. The bait was checked each day to detect the presence of eggs. On 23 August (day 12), the first eggs were found (about 50). They were left for 6 days, corresponding to the same interval of 18 days between the disappearance of the victim and his discovery, and then transported and Ieft another 3 days in the cold room at the institute. Afterward, eggs were collected and reared as mentioned above. The first eggs hatched the same day, corresponding to day 21. All emerged adults belonged to C. vicina.

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21

Days

Fig. I. Succession of the main events.

Comparing both set of data, we have good evidence that the man died the day he was reported missing (8/7/97) or 1 day later. This assumption is sup­ported on the following bases. First, according to Davies and Ratcliffe (1994), eggs of C. vicina need about 9 days at 5°C to hatch. We can reasonably assume that the mean temperature supported by eggs during the case study was about 5-5.5°C. Therefore from 28 July (hatching day), we cut off 9 days and estimated the egg-laying day as the 19th. Second, in our experiment 12 days was needed to attract blowflies to the hait. This delayed attractivity of our bait is due partly to the low-temperature limiting decomposition. Cutting 12 days off the egg-lay­ing day we get 8 July. This is exactly what the results of our experimental case showed.

This brings new limits to forensic entomology because, as mentioned pre­viously, it was generally assumed that blowflies did not fly and lay eggs under 10-12°C and normally not in total darkness. But females were nevertheless

 

attracted to the corpse or the bait, coming from outside and flying downward about IO m until they reached the substrate. We suppose that the higher metabolic rate of C. vicina and the heat stored by sunlight could explain the possibility of entering a cold cave to oviposit. However, in a sertes of experiments done under laboratory conditions with C. vicina, when females (collected in winter) were left at different temperatures, ranging from 5 to 21°C, they stopped ovipositing at about 8°C (unpublished).

ACKNOWLEDGMENTS

We would like to express our thanks to Dr. B. Merz for comments on the manuscript.

REFERENCES

Catts, E. P. (1992). Problems in estimating the postmortem interval in death investigations. J. Agr. EntomoL 9: 245-255.

Davies, L., and Ratcliffe. G. G. (1994). Development rates of some pre-adult stages in blowflies with reference to low temperatures. Med. Vet Ent. 8: 245-254.

Deonier, C. C. (1940). Carcass temperatures and their relation to winter blowfly populations and activity in the Southwest. J. Econ. EntomoL 33: 166-170.

Erzinçlioglu, Y. Z. (1996). Blowflies, Naturaliste Handbook 23, Richmond.

Green, A. A. (1951). The control of blow files infesting slaughter bouses. 1. Field observations of the habits of blow flics. Ann. AppL Bial. 38: 475-494.

Greenberg, B. (1973). Flies and Disease, Vol. 2, Princeton University Press, Princeton, NJ. Greenberg, B. (1985). Forensic entomology: Case studies. Bull. EntomoL Soc. Am. 31: 25-28. Greenberg, B. (1990). Noctural oviposition behavior of blow flics (Diptera: Calliphoridae). J. Med.

EntomoL 27: 807-810.

Greenberg, B. (1991). Flics as forensic indicators. J. Med. EntomoL 28: 565-577.

Meyer, S. G. E., and Schaub, G. (1973). Der respiratorische Stoffwechsel von Calliphoridenlarven

in Beziehung zu Temperaturadaption und Regulation. J. Insect Physiol. 19: 2183-2198.
Nielsen, B. O., and Nielsen, S. A. (1946). Schmeissfliegen (Calliphoridae) und vakuumverpackter

Schinken. Anzeiger Schadlingsk. Pflanzen Umweltschutz 49: 113-115.

Nuorteva, P. (1959). Studies on the significance of flics in the transmission of poliomyelitis. The composition of the blowfly fauna in different parts of Finland during the year 1958. Ann. Ento­mol. Fenn. 25: 137-162.

Nuoneva, P. (1965). The flying activity of blowflies (Dipt., Calliphoridae) in subarctic conditions. Ann. Entomol. Fenn. 31: 242-245.

Rognes, K. (1991). Blowflies (Diptera, Calliphoridae) of Fennoscandia and Denmark, Vol. 24, E. J. Brill/Scandinavian Science Press.

Williams, H. (1984). A model for the aging of fly larvae in forensic entomology. Forens. Sel. Int. 25: 191-199.

Wyss, C. (1997). Forensic entomology in Lausanne (CH). Oistros 5: 2-5.

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